NAD+ 1000mg Dosage Protocol | PeptideDosages.com

NAD+ (1000 mg Vial) Dosage Protocol

NAD+ Dosage Chart

NAD+ is dosed at 50 mg–100 mg daily via subcutaneous injection in educational protocols. A 1000 mg vial reconstituted with bacteriostatic water yields about 333.3 mg/mL. This information is for research and educational use only.

Reconstitute: Add 3.0 mL bacteriostatic water → 333.3 mg/mL concentration.

Typical daily range: 50–100 mg once daily subcutaneously (gradual titration from lower doses).

Easy measuring: At 333.3 mg/mL, 1 unit = 0.01 mL = 3.33 mg on a U‑100 insulin syringe.

Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 14 days; protect from light and avoid freeze–thaw cycles.

NAD+ (nicotinamide adenine dinucleotide) is a critical coenzyme involved in cellular energy metabolism, DNA repair, and mitochondrial function[1]. Clinical research has primarily employed intravenous infusions at high doses, though subcutaneous administration at lower doses is emerging as a practical maintenance route[2][3]. This educational protocol presents a once‑daily subcutaneous approach with gradual titration for improved tolerability.

Research context: For evidence on mechanisms, human and preclinical research, limitations, and safety, read NAD+ Peptide: Benefits, Uses, Side Effects, Dosage, and Research.

Standard / Gradual Titration Approach (3 mL = 333.3 mg/mL)

Week 1

50 mg

15 units (0.15 mL)

Week 2

75 mg

22.5 units (0.225 mL)

Weeks 3–8

100 mg

30 units (0.30 mL)

Weeks 9–12

Weeks 13–16

Frequency: Inject once daily subcutaneously. This gradual titration protocol begins at 50 mg daily to assess tolerance, as starting too high can produce adverse reactions such as insomnia, anxiety, or fatigue[4]. Most individuals find doses in the 50–100 mg range well‑tolerated after ramp‑up[5]. The 3.0 mL dilution keeps per‑injection volumes practical and allows for accurate unit measurements on standard insulin syringes.

Reconstitution Steps

Allow the lyophilized vial to reach room temperature before opening to minimize moisture condensation.

Draw 3.0 mL bacteriostatic water (0.9% benzyl alcohol) with a sterile syringe.

Inject slowly down the vial wall to avoid foaming; do not aim directly at the powder.

Gently swirl or roll the vial until the powder fully dissolves (do not shake vigorously).

The resulting solution should be clear and colorless. If discoloration or precipitate appears, discard.

Label with the reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Use within 14 days; inspect before each use for clarity.

Note: Each 0.01 mL (1 unit) contains approximately 3.33 mg of NAD+. Example conversions: 50 mg = 15 units; 75 mg = 22.5 units; 100 mg = 30 units.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration as outlined above.

Peptide Vials (NAD+, 1000 mg each):

8 weeks ≈ 6 vials (total 5,075 mg used)

12 weeks ≈ 8 vials (total 7,875 mg used)

16 weeks ≈ 11 vials (total 10,675 mg used)

Insulin Syringes (U‑100, 1 mL):

Per week: 7 syringes (1/day)

8 weeks: 56 syringes

12 weeks: 84 syringes

16 weeks: 112 syringes

Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution.

8 weeks (6 vials): 18 mL → 2 × 10 mL bottles

12 weeks (8 vials): 24 mL → 3 × 10 mL bottles

16 weeks (11 vials): 33 mL → 4 × 10 mL bottles

Alcohol Swabs: One for the vial stopper + one for the injection site each day.

Per week: 14 swabs (2/day)

8 weeks: 112 swabs → recommend 2 × 100‑count boxes

12 weeks: 168 swabs → recommend 2 × 100‑count boxes

16 weeks: 224 swabs → recommend 3 × 100‑count boxes