NAD+ Dosing: 100mg SubQ, 2-3x/Week Protocol (2026)

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme present in every living cell, essential for mitochondrial energy production, DNA repair, and sirtuin activation. NAD+ levels decline approximately 50% between ages 40 and 60 (Massudi et al., 2012), driving interest in direct supplementation to restore youthful concentrations.

Research-context information only. NAD+ is a research peptide. Protocols, doses, and reactions reported below come from published research and self-reported community sources. This article reports what has been documented, not what should be done. Consult a licensed physician for personal medical decisions.

NAD+ is not FDA-approved as an anti-aging therapy. The protocols below reflect published research, clinical practice patterns, and community experience. This is not medical advice.

NAD+ Dosing Table

Match your vial size below — reconstitution and dose math update automatically.

50 mg

50 units

0.5 mL

2-3x/week SubQStarter

100 mg

100 units

1 mL

2-3x/week SubQStandard

200 mg

200 units

2 mL

2x/week SubQAdvanced

Math assumes U-100 insulin syringes (1 mL = 100 units). Verify your syringe matches before injecting. Round half-units to the nearest visible mark.

2-3x/week SubQ

2x/week SubQ

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Quick Reference: Standard Protocol

Vial

500 mg

BAC Water

5 mL

Concentration

100 mg/mL

Dose

100 mg (100 units on insulin syringe)

Route

Subcutaneous

Timing

AM

Frequency

2-3 days per week

Cycle

As needed

Storage

Refrigerate, use within 28 days

NAD+ Dosing by Route

NAD+ is unique among peptides in having five practical administration routes, each with different onset and bioavailability:

Subcutaneous injection

100-200 mg

2-3x per week

Days

High (direct)

IV infusion

250-500 mg

1-2x per month

Hours

Highest

Sublingual

50-100 mg

Daily

1-2 weeks

Moderate

Nasal spray

25-50 mg

Oral NMN

250-1000 mg

2-4 weeks

Indirect

Oral NR

300-1000 mg

Cycling Details

NAD+ doesn't require strict cycling like most peptides. The "as needed" approach reflects that NAD+ is a coenzyme, not a receptor agonist — there's no desensitization concern in the same way.

Community protocols most commonly describe an 8-12 week injectable course (100-200 mg SC 2-3x/week), with oral NMN (500 mg daily) as a bridge during off periods before repeating.

Routes of Administration

Subcutaneous injection is the route community and clinical protocols most commonly use as the primary injectable route. Injection site stinging lasting 5-15 minutes is the most frequently reported side effect; community sources describe slow injection and site rotation as common approaches. Community sources most often describe morning timing, citing potential energizing effects.

IV infusion (250-500 mg over 2-4 hours) delivers the highest peak levels and is used in clinical settings. A single 3-hour infusion of 750 mg increased blood NAD+ approximately 398% above baseline (Grant et al., 2019). Loading protocols use 250-500 mg daily for 3-5 days.

Oral precursors (NMN/NR) are the most accessible route. NMN at 250 mg/day for 12 weeks significantly increased NAD+ metabolites and improved physical performance (Yoshino et al., 2021). NR at 1000 mg/day increased whole-blood NAD+ by ~60% over 6 weeks (Martens et al., 2018).

Reconstitution Quick Reference

200 units (split)

Math (as described in community reconstitution protocols): 500 mg ÷ 5 mL = 100 mg/mL. For a 100 mg dose: 100 ÷ 100 = 1.0 mL = 100 units on an insulin syringe.

Protocols describe refrigerating after mixing and using within 28 days. NAD+ is less stable in solution than many peptides, so prompt refrigeration matters. For step-by-step instructions, see the full NAD+ Reconstitution Guide.