Semax Dosing: 1mg SubQ, 2-3x/Week Protocol (2026)

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Reconstitution Quick Reference

30 mg

3 mL

10 mg/mL

10 units

Math: 30 mg / 3 mL = 10 mg/mL. For 1 mg: 1 / 10 = 0.1 mL = 10 units.

Community protocols describe gentle swirling — not shaking — to avoid degradation, with storage at 2–8°C and use within 28 days.

Where These Numbers Come From

Semax has a substantial clinical evidence base from Russian research institutions.

Dopaminergic and serotonergic activation: Semax activates brain dopamine and serotonin systems, explaining its nootropic and mood-enhancing effects (Eremin et al., 2005).

BDNF upregulation: A single intranasal dose increases BDNF protein levels 1.4-fold and BDNF mRNA 3-fold in the rat hippocampus, with concurrent trkB receptor activation (Dolotov et al., 2006).

Stroke neuroprotection: Clinical evaluation as a neuroprotective agent in acute ischemic stroke at 100-150 mcg/kg doses, with angioprotective and neurotrophic activity (Gusev et al., 1999). Semax suppresses proinflammatory mediator transcription during brain ischemia (Dergunova et al., 2021).

Stacking Protocols

Semax + Selank

Classic Russian stack — focus + calm

Both 1 mg SC, AM, 2-3x/week

Semax + BPC-157

Neuroprotective — BDNF + dopamine repair

Semax 1 mg + BPC-157 500 mcg

Semax + SS-31

Brain energy — neuroplasticity + mitochondria

Semax 1 mg 2-3x/week + SS-31 500 mcg 5on/2off

Overstimulation is reported when Semax is combined with caffeine or other stimulants. Community sources describe keeping stimulating stacks to the first half of the day.

Side Effects & Safety

Low toxicity — extensive preclinical and clinical testing in Russia

No hormonal effects — unlike full ACTH, Semax does not stimulate cortisol or adrenal activity

No tolerance documented — Russian clinical trial data report consistent efficacy across documented course durations

Mild nasal irritation — intranasal route

Slight headache — particularly first 1-2 days

Mild insomnia — if dosed too late in the day

Hair loss — community sources describe rare reports at elevated doses, speculatively attributed to melanocortin receptor activity

Clinical exclusion criteria (from Russian trial protocols): Pregnancy/breastfeeding, active malignancy (BDNF/growth-factor interactions noted), acute psychosis (dopaminergic stimulation). Trial protocols excluded subjects with these profiles.

mg to Units Conversion

On a standard 100-unit insulin syringe, each "unit" equals 0.01 mL (so 100 units = 1 mL). Once Semax is reconstituted, the conversion from a target dose to syringe units depends on the chosen dilution.

The two reconstitution ratios most often described in community protocols are below.

Reconstitution A: 30 mg vial + 3 mL BAC water (10 mg/mL) — the standard dilution from the Quick Reference above.

0.5 mg

0.05 mL

5 units

1 mg

0.1 mL

1.5 mg

0.15 mL

15 units

2 mg

0.2 mL

20 units

Reconstitution B: 30 mg vial + 5 mL BAC water (6 mg/mL) — more BAC water for larger, easier-to-measure draws.

0.083 mL

8.3 units

0.167 mL

16.7 units

0.25 mL

25 units

0.333 mL

33.3 units

These conversions reflect the dilutions documented in community reconstitution protocols. They report how the math is described, not a recommended dosing schedule.

Core Supplies for This Protocol

The three essentials for running any reconstituted injectable: cold storage, accurate syringes, and metabolic tracking.

Cooluli Classic 4L Mini Fridge

Compact thermoelectric mini-fridge with heat/cool toggle. The most-mentioned dedicated peptide-storage fridge in research community sources — fits ~20 vials and runs quietly.

BD Ultra-Fine 31G 0.3cc 5/16" Insulin Syringes (Box of 90)

0.3cc 31G syringes — each gradation marks 1 unit (vs 2 units on 1cc), making sub-50-unit peptide doses accurate. BD Ultra-Fine is the most widely-cited brand in peptide community sources.

CONTOUR NEXT GEN Glucose Meter All-In-One Kit

Ascensia's CONTOUR NEXT GEN — the most clinically-validated home glucose meter. Includes 20 test strips + lancing device. Tracks the blood-sugar response GLP-1 users care about, especially during dose escalation.

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