Thymosin Alpha-1 Dosing: 1.5mg 5on/2off Protocol (2026)
Affiliate disclosure: vendor links in this article are affiliate links — The Peptide Catalog may earn a commission if you buy through them, at no additional cost to you. Where These Numbers Come From Thymosin alpha-1 has one of the strongest clinical evidence
Affiliate disclosure: vendor links in this article are affiliate links — The Peptide Catalog may earn a commission if you buy through them, at no additional cost to you.
Where These Numbers Come From
Thymosin alpha-1 has one of the strongest clinical evidence bases of any peptide in the research community.
Immune Modulation: A comprehensive review confirmed thymosin alpha-1's pleiotropic effects on T-cells, NK cells, dendritic cells, and macrophages — restoring immune function across diverse clinical settings (Tuthill et al., 2016).
Hepatitis B Trials: Thymalfasin was extensively studied at 1.6 mg twice weekly for 6-12 months, showing improved virological response and enhanced vaccine responsiveness in immunocompromised patients.
Sepsis Data: A large Phase III trial (TESTS) evaluated thymosin alpha-1 at 1.6 mg in sepsis patients, providing significant data on immune modulation in critical illness (Li et al., 2025). An earlier meta-analysis showed trends toward reduced mortality from multiple organ failure (Li et al., 2015).
Safety Review: A narrative review covering 11,000+ subjects across 30+ trials confirmed an excellent safety profile with no dose-limiting toxicities (Dinetz et al., 2024).
Stacking Protocols
Thymosin Alpha-1 + LL-37 (Immune Defense Stack)
Thymosin alpha-1
1.5 mg
SC
5on/2off, AM
Adaptive immune modulation (T-cells, NK cells)
LL-37
50-100 mcg
Daily or 3x/week
Innate immune defense (antimicrobial)
Thymosin Alpha-1 + Thymulin
Broad immune modulation
Thymulin
2 mg
PM
T-cell maturation (zinc-dependent)
Complementary thymic peptides — TA-1 acts downstream on function, thymulin acts upstream on development.
Thymosin Alpha-1 + BPC-157
Immune support
BPC-157
250-500 mcg
Daily
Tissue repair, gut healing
Side Effects & Safety
Injection site reactions — mild erythema, minor discomfort (most common, transient)
No dose-limiting toxicities identified across 30+ clinical trials
No significant organ toxicity reported in 11,000+ subjects
No immune overstimulation — modulates rather than simply stimulates immunity
Safe in immunocompromised — extensively used in HIV, hepatitis, and cancer patients
Autoimmune conditions: Clinical context — trials have not specifically studied this population; immune modulation effects may warrant clinical monitoring
Organ transplant: Clinical context — immune enhancement could theoretically affect transplant tolerance; not studied in transplant populations
mg to Units Conversion
On a standard 100-unit insulin syringe, each "unit" equals 0.01 mL (so 100 units = 1 mL). Once thymosin alpha-1 is reconstituted, the conversion from a target dose to syringe units depends on the chosen dilution.
The two reconstitution ratios most often described in community protocols are below.
Reconstitution A: 10 mg vial + 2 mL BAC water (5 mg/mL) — the standard dilution from the Quick Reference above.
1 mg
0.2 mL
20 units
0.3 mL
30 units
0.4 mL
40 units
3 mg
0.6 mL
60 units
Reconstitution B: 10 mg vial + 1 mL BAC water (10 mg/mL) — less BAC water for a lower total volume, so smaller draws and less fridge space.
0.1 mL
10 units
0.15 mL
15 units
These conversions reflect the dilutions documented in community reconstitution protocols. They report how the math is described, not a recommended dosing schedule.
Core Supplies for This Protocol
The three essentials for running any reconstituted injectable: cold storage, accurate syringes, and metabolic tracking.
Cooluli Classic 4L Mini Fridge
Compact thermoelectric mini-fridge with heat/cool toggle. The most-mentioned dedicated peptide-storage fridge in research community sources — fits ~20 vials and runs quietly.
BD Ultra-Fine 31G 0.3cc 5/16" Insulin Syringes (Box of 90)
0.3cc 31G syringes — each gradation marks 1 unit (vs 2 units on 1cc), making sub-50-unit peptide doses accurate. BD Ultra-Fine is the most widely-cited brand in peptide community sources.
CONTOUR NEXT GEN Glucose Meter All-In-One Kit
Ascensia's CONTOUR NEXT GEN — the most clinically-validated home glucose meter. Includes 20 test strips + lancing device. Tracks the blood-sugar response GLP-1 users care about, especially during dose escalation.
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