Source comparison
BPC-157 Research Time Zone Considerations: Protocol Comparison
Dosing Schedule Fixed clock times (e.g., 08:00, 20:00 local) Circadian-phase-matched times relative to DLMO at each site Not directly temperature-dependent Multi-zone requires actigraphy or sleep logs; single-site can use fixed clock times without circadian co
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- Dosing Schedule
- Fixed clock times (e.g., 08:00, 20:00 local)
- Circadian-phase-matched times relative to DLMO at each site
- Not directly temperature-dependent
- Multi-zone requires actigraphy or sleep logs; single-site can use fixed clock times without circadian correction
- Peptide Shipment
- Direct lab-to-fridge transfer, minimal transport time
- Validated cold-chain shipping with continuous temperature logging
- Lyophilised: ≤25°C max 72h; Reconstituted: 2–8°C continuously
- Ship lyophilised powder to reduce temperature risk; reconstitute on-site per standardised SOP
- Reconstitution Timing
- Can batch-prepare for full study cohort if used within 28 days
- Must coordinate prep timing across sites to minimise storage duration variability
- Post-reconstitution: 2–8°C storage, use within 28 days
- Stagger reconstitution so all sites use peptide within same degradation window (e.g., days 1–7 post-mixing)
- Plasma Sampling Windows
- Consistent intervals relative to dose administration
- Must account for time-zone-shifted circadian phase when comparing peak/trough PK
- Sample cold chain same as peptide transport
- Draw samples at matched circadian phases, not matched UTC times. 'morning sample' means same hours-post-wake across all sites
- Data Aggregation
- Timestamps in single local time zone
- Requires conversion to circadian time or UTC with phase annotation
- Not temperature-dependent
- Log both local clock time AND hours-relative-to-wake for every dose and measurement. Allows post-hoc circadian adjustment