Source comparison
CJC-1295 No DAC Gene Expression: Research Model Comparison
Rodent (in vivo) MyoD, myogenin, IGF-1, PGC-1α RT-PCR, Western blot Peak 18–36 hours, baseline by 72 hours Species-specific GH receptor differences limit direct human translation Human myotube culture (in vitro) MRF4, myogenin, GLUT4 RNA sequencing, immunofluo
This comparison does not assign a generated winner or score.
- Rodent (in vivo)
- MyoD, myogenin, IGF-1, PGC-1α
- RT-PCR, Western blot
- Peak 18–36 hours, baseline by 72 hours
- Species-specific GH receptor differences limit direct human translation
- Human myotube culture (in vitro)
- MRF4, myogenin, GLUT4
- RNA sequencing, immunofluorescence
- Peak 24 hours, decline by 48 hours
- Lacks systemic GH-IGF-1 axis. Direct IGF-1 addition required to simulate in vivo effects
- Porcine skeletal muscle biopsy
- IGF-1, SOCS2, STAT5 phosphorylation
- Microarray, qPCR
- Detectable at 6 hours, sustained through 48 hours
- Biopsy timing variability introduces measurement noise
- Primate (non-human)
- Hepatic IGF-1, muscle MyoD
- Tissue biopsy with RNA extraction
- Consistent with rodent models but delayed peak (24–48 hours vs 18–24 hours)
- Ethical and cost constraints limit sample size and longitudinal data