Source comparison
How Concentrated Should SS-31 Be for Research?: Model Type Comparison
Isolated cell monolayer (2D culture) 0.5–5 µM (0.5–5 mg/mL) 1–48 hours Direct addition to culture media Standard concentration range validated across multiple cell types; start at 1 µM for initial dose-response studies 3D cell culture (spheroids, organoids) 5–
This comparison does not assign a generated winner or score.
- Isolated cell monolayer (2D culture)
- 0.5–5 µM (0.5–5 mg/mL)
- 1–48 hours
- Direct addition to culture media
- Standard concentration range validated across multiple cell types; start at 1 µM for initial dose-response studies
- 3D cell culture (spheroids, organoids)
- 5–20 µM (5–20 mg/mL)
- 4–72 hours
- Media perfusion or direct addition
- Higher concentration compensates for diffusion gradients; monitor spheroid core viability independently
- Tissue explants (organ slices)
- 10–50 µM (10–50 mg/mL)
- 2–24 hours
- Immersion in oxygenated buffer
- Tissue architecture limits penetration; consider slice thickness when selecting concentration
- Isolated perfused organs (ex vivo)
- 50–100 µM (50–100 mg/mL)
- 30 min – 2 hours
- Perfusate addition (recirculating)
- Highest concentration required due to whole-organ distribution; verify coronary flow rates to ensure delivery
- In vivo studies (reference only)
- 3–10 mg/kg body weight (IV or IP)
- Single dose or daily for 7–28 days
- Systemic injection
- Not a concentration per se but demonstrates required dosing for whole-organism effects; consult institutional guidelines