Source comparison
How to Calculate Thymosin Alpha-1 Concentration: Full-Spectrum Comparison
Peptide Mass Source Use vial label mass directly Multiply label mass by COA purity % to get true mass Assuming label = actual content Ignoring purity creates 2–15% dosing error. COA is non-negotiable for accurate concentration Molecular Weight 3,108.3 Da (thym
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- Peptide Mass Source
- Use vial label mass directly
- Multiply label mass by COA purity % to get true mass
- Assuming label = actual content
- Ignoring purity creates 2–15% dosing error. COA is non-negotiable for accurate concentration
- Molecular Weight
- 3,108.3 Da (thymosin alpha-1)
- Same. Confirm sequence matches before calculation
- Using thymosin beta-4 MW (4,963 Da) by mistake
- Molecular weight error cascades through molar conversions. Verify sequence identity first
- Solvent Volume
- 2.0 mL bacteriostatic water (standard convenience volume)
- 5.0 mL for doses requiring 0.2–0.5 mL injection volumes
- Adding solvent without accounting for vial headspace pressure
- Higher volumes improve dose measurement precision but require larger injection volumes
- Concentration Units
- mg/mL (simpler for manual calculation)
- μg/mL (matches protocol dose specifications directly)
- Mixing units mid-calculation (e.g., mg dose ÷ μg/mL concentration)
- Express everything in matching units before dividing. Unit mismatch is the #1 calculation error
- Dosing Volume Calculation
- Target dose (μg) ÷ concentration (μg/mL)
- Same, but round to syringe precision (0.01 mL)
- Calculating volume to excessive decimal places the syringe can't measure
- Rounding to equipment precision prevents false accuracy. 0.3267 mL rounds to 0.33 mL for insulin syringes
- Documentation
- Write concentration on vial label
- Record label mass, purity %, true mass, solvent volume, final concentration, and reconstitution date
- Relying on memory across multi-week studies
- Labeling prevents dose drift when multiple researchers access the same vial