Source comparison
Melanotan-1 Concentration Comparison by Assay Type
Radioligand Binding (IC50 Determination) 0.1 nM – 10 µM (10-point curve) 100–200 µL per well All MC receptors (non-selective) Use concentrations spanning 3 logs above and below expected IC50. For MC1R this means starting at 0.1 nM cAMP Functional Assay (EC50)
This comparison does not assign a generated winner or score.
- Radioligand Binding (IC50 Determination)
- 0.1 nM – 10 µM (10-point curve)
- 100–200 µL per well
- All MC receptors (non-selective)
- Use concentrations spanning 3 logs above and below expected IC50. For MC1R this means starting at 0.1 nM
- cAMP Functional Assay (EC50)
- 0.1 nM – 100 nM (8-point curve)
- 50–100 µL per well
- MC1R, MC3R, MC4R (subtype-dependent)
- Keep maximum concentration below 1 µM to preserve receptor subtype specificity in mixed-receptor cell lines
- Melanogenesis Assay (Melanocyte Culture)
- 10 nM – 1 µM (single-dose or dose-response)
- 500 µL – 2 mL per well (6–24 well format)
- MC1R (primary)
- Maximal melanin synthesis typically observed at 100 nM. Higher concentrations produce no additional effect
- Murine In Vivo (Subcutaneous Injection)
- 1–5 mg/mL stock; dose 0.5–2 mg/kg
- 5–50 µL per injection (25 g mouse)
- Systemic (all MC receptors)
- Prepare fresh stock weekly. Peptide degradation in reconstituted solution reduces effective dose by 10–15% after 7 days at 4°C
- Non-Human Primate Dermatology
- 2–10 mg/mL stock; dose 0.1–0.5 mg/kg
- 100–500 µL per injection
- MC1R (dermal melanocytes)
- Higher concentrations allow smaller injection volumes but require aggregation testing via light scattering before use