Source comparison
Physicochemical and pharmacokinetic comparison
TB-500 (Thymosin Beta-4 intact peptide) has a molecular weight of approximately 4964Da (43 amino acids), an isoelectric point of approximately 5.0 (acidic), and is water-soluble at typical research concentrations (1–2mg/mL in sterile water or PBS). It is stabl
This comparison does not assign a generated winner or score.
- TB-500 (Thymosin Beta-4 intact peptide) has a molecular weight of approximately 4964Da (43 amino acids), an isoelectric point of approximately 5.0 (acidic), and is water-soluble at typical research concentrations (1–2mg/mL in sterile water or PBS). It is stable lyophilised at −20°C for approximately 24 months, and reconstituted solutions retain activity at 4°C for approximately 7–14 days in the absence of carrier protein. Susceptibility to oxidation at methionine residues (Met6) requires care with reconstitution conditions — avoid repeated freeze-thaw cycles, which progressively oxidise the methionine to sulfoxide, reducing G-actin binding affinity approximately 30–40%.
- Follistatin FST-315 (recombinant, expressed in mammalian HEK293 or CHO cells to ensure correct glycosylation) has a core molecular weight of approximately 35kDa, glycosylated to approximately 40–65kDa. Glycosylation is essential for correct folding and activin-A binding geometry — bacterially expressed FST lacks glycosylation and shows approximately 3–5-fold lower activin-A binding affinity and faster systemic clearance. Research-grade FST-315 is typically supplied at ≥95% purity by RP-HPLC with confirmation of bioactivity via pSmad2 suppression assay in A204 rhabdomyosarcoma cells. Lyophilised FST-315 is stable at −80°C for approximately 12 months; reconstituted solutions should be used within 24–48 hours or snap-frozen in single-use aliquots at −80°C.