Source comparison
TB-500 In Vitro Research: Model Comparison
Scratch Assay (Fibroblasts) Wound closure rate, cell migration speed 10–50 µg/mL 50–70% faster closure vs control; peak effect at 48–72 hours 2D model doesn't replicate 3D tissue architecture; no immune component Gold standard for directional migration. Fast,
This comparison does not assign a generated winner or score.
- Scratch Assay (Fibroblasts)
- Wound closure rate, cell migration speed
- 10–50 µg/mL
- 50–70% faster closure vs control; peak effect at 48–72 hours
- 2D model doesn't replicate 3D tissue architecture; no immune component
- Gold standard for directional migration. Fast, reproducible, but oversimplified
- Transwell Migration (Endothelial Cells)
- Chemotactic migration through porous membrane
- 20–100 µg/mL
- 200–400% increase in migrated cell count; VEGF-independent effect
- Doesn't assess cell-cell adhesion or matrix remodelling
- Best for isolating chemotaxis; limited functional angiogenesis data
- Matrigel Tube Formation (HUVECs)
- Tubular network formation, branch points
- 60% faster tube formation; 40% more branch points
- Matrigel composition variability; lacks flow dynamics
- Closest in vitro proxy for angiogenesis. Clinically relevant but material-dependent
- Macrophage Polarisation (Bone Marrow-Derived)
- M1/M2 marker expression (CD206, iNOS, arginase-1)
- 150–200% increase in M2 markers; 40–60% reduction in M1 markers
- Doesn't replicate tissue-resident macrophage heterogeneity
- Strong mechanistic insight into inflammation modulation. But polarisation is context-dependent in vivo
- MSC Differentiation (Osteogenic/Chondrogenic)
- Lineage-specific marker expression (Runx2, Sox9, collagen II)
- 50–200 µg/mL
- Enhanced response to differentiation media; no spontaneous differentiation
- High donor variability in primary MSCs; media composition strongly influences outcome
- Suggests TB-500 amplifies lineage commitment signals. Not a standalone differentiation trigger