Source comparison
Verify Tesamorelin + Ipamorelin Blend Purity: HPLC vs MS Comparison
HPLC with UV Detection Relative purity (%) by peak area 0.1% impurities 2–4 hours $150–$250 Peptide separation, concentration ratio, impurity percentage Exact molecular identity, sequence errors, oxidation state ESI-MS Molecular weight (Da) ±0.5 Da mass accura
This comparison does not assign a generated winner or score.
- HPLC with UV Detection
- Relative purity (%) by peak area
- 0.1% impurities
- 2–4 hours
- $150–$250
- Peptide separation, concentration ratio, impurity percentage
- Exact molecular identity, sequence errors, oxidation state
- ESI-MS
- Molecular weight (Da)
- ±0.5 Da mass accuracy
- 1–2 hours
- $200–$350
- Amino acid sequence correctness, oxidation products, truncations
- Quantitative concentration, enantiomeric purity
- Amino Acid Analysis
- Molar ratio of each amino acid
- 0.5% composition variance
- 24–48 hours
- $300–$450
- Substitution errors, hydrolysis-stable modifications
- Labile modifications (phosphorylation, acetylation)
- LAL Endotoxin Assay
- Bacterial endotoxin contamination (EU/mg)
- 0.01 EU/mL
- 48 hours
- $80–$150
- Sterility of synthesis and lyophilisation process
- Chemical purity, peptide concentration
- Bottom Line
- HPLC + ESI-MS together provide the minimum verification standard for research-grade peptide blends. HPLC confirms purity percentage and ratio accuracy, while MS confirms molecular identity and detects degradation products. AAA and endotoxin testing are required for regulatory submissions or when working with novel sequences.