Source comparison
TB-500 Research Lab Test Recommendations: Test Comparison
Before selecting a testing protocol, compare the three core verification methods based on what each reveals, acceptable thresholds, turnaround time, and cost per sample. This table summarizes the decision criteria for HPLC, mass spectrometry, and endotoxin tes
This comparison does not assign a generated winner or score.
- Before selecting a testing protocol, compare the three core verification methods based on what each reveals, acceptable thresholds, turnaround time, and cost per sample. This table summarizes the decision criteria for HPLC, mass spectrometry, and endotoxin testing.
- HPLC (High-Performance Liquid Chromatography)
- Peptide purity and concentration vs synthesis impurities
- ≥95% purity; concentration within ±10% of label claim
- 3–5 business days
- $150–$300
- Required for every batch. This is your first-line verification that you have TB-500 and not a mislabeled vial
- Mass Spectrometry (LC-MS or MALDI-TOF)
- Exact molecular weight confirmation; detects synthesis errors, oxidation, truncation
- Measured MW within ±2 Da of theoretical 4963.44 Da
- 5–7 business days
- $200–$400
- Non-negotiable for mechanism studies or when receptor binding is critical. Catches structural problems HPLC misses
- LAL Endotoxin Testing (Limulus Amebocyte Lysate)
- Bacterial endotoxin contamination from gram-negative bacteria
- <0.5 EU/mL for cell culture; <10 EU/mL for animal models
- 1–2 business days
- $75–$150
- Required before any in vivo or cell culture use. Endotoxin triggers immune responses that confound experimental results